LOCUS       Tn6732a                 7099 bp    DNA     linear       29-SEP-2020
DEFINITION  Leclercia adecarboxylata.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Leclercia adecarboxylata
  ORGANISM  Leclercia adecarboxylata
            Unclassified.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of Tn6732a
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Leclercia adecarboxylata"
                     /mol_type="genomic DNA"
     repeat_region   complement(1..23)
                     /gene="IRR_IS1F"
     CDS             complement(16..519)
                     /gene="insB"
                     /codon_start=1
                     /product="IS1F transposase InsB"
                     /translation="MSRQCTHYGRWPQHGFTSLKKLRPQSVTWRIQPGSDVIVCAEMD
                     EQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDG
                     WPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
                     LNIKHYQ"
     CDS             complement(438..713)
                     /gene="insA"
                     /codon_start=1
                     /product="IS1F transposase InsA"
                     /translation="-
                     ASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDM
                     AMNGVGCRASARIMGVGLNTVLRHLKNSGRSR"
     repeat_region   complement(746..768)
                     /gene="IRL_IS1F"
     CDS             962..1927
                     /codon_start=1
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /translation="MSKKMTMAEISRQLGISTMTVSRYFNGGYVSEENRLKIDAIVKE
                     SHYTPNIFARSIRSQSNIIGFIAPRIESYTTSLVIKGALAAANESQVRMLFHATGFNH
                     ESECQAVREFNGLNALGTIIIASKHSVEETFYRTLDNVVFIGKNTPHHCCLYYPDHAA
                     IKALVSHTITQLKQQQAPLAAVHYIYDERMLSSRTKLMQATITDTVPELNLSLQALAN
                     SEDKAGYQAVQLQPNHVYFCATDNIAIRLYRRAKEQQLKIGHNVWIVGMGDYDYSDLL
                     VPSLTTVAFNYYQVGYQAVKKLLKRDFSSVEGTFDIKMRESSQFL"
     CDS             1996..3729
                     /codon_start=1
                     /product="Alpha-glucosidase"
                     /translation="MTLAPNMHSKPLAWWKKATAYQIYPRSFYDTNQDGIGDINGIIA
                     KLDYLADLGIDLIWICPFYASPNDDNGYDISDYQAIHPDFGTLEDVDELISAAHKRGI
                     RIIMDLVINHTSDEHPWFIESRDNKNSPKRDWYIWRDGCEPTAEKPTCDPNNWESIFH
                     GSAWEYDKKTAQYYLHLFSKKQPDLNWENPEVKQALFKMIYWWLERGIDGFRVDAISH
                     IQKQPGLPSLPNPKGEKYVSSFDYHMNVAGIEKHLHELKEQAFDPFNIVTVGEANGVT
                     ADNALLWVAEATEHDQGGVFNMIFQFEHMKLWSEEQSNVPLDLVEFKRILSRWQDALE
                     GKGWNALYLENHDQARSIDTFADPTSRYASATALASCYFLMKGTPFIYQGQEIGMVNH
                     QFTSLDEFNDVSARNLIQKLSQQGQSDADILALLNQVSRDHSRLPMQWNAQDFAGFSE
                     VQPWFSVNQQYADINVEQQQKDPNSILSFYKQLIALRKSNVSLVVGRYQLLLPNDPNI
                     YAYQRVAQDMTWTIITNLSPQESIVDIDRMQLGELMLDNQNVNNEHVRSDFIATQIAP
                     PYAAYIFARKH"
     CDS             3789..5417
                     /gene="orf1629"
                     /codon_start=1
                     /product="PTS transporter subunit IICB"
                     /translation="MSQLLSFDFWQRFGKSLIVVIAVMPAAGIMISLGKVVAMYAGGI
                     GAIETLGAIMENIGWGIIVNLHLLFAVAIGGSWAKERAGGAFAALIAFILINRITGVI
                     LGVDNAMLSDPEAVVMSLFGSELPVSQFFTNILGAPALNMGVFIGIMSGYLGANLFNR
                     YHDFARLPQALAFFNGKRFVPFVVIFYSMIIAFALSIVWPLIQGALNDFGQWIATSKD
                     TAPITAPFLYGTLERLLLPFGLHHTLTIPMNYTELGGTYELLTGVNAGASVYGQDPLW
                     LAWVSDLNNLKLSDPTTYQHLLDTVHPARFKAGQVIIAASSLIGIGLAMYHCVDSDKR
                     AQYKPMFLSACLAVLLTGVTEPIEFMFMFIAPVLYVAHAVLTGIAFALVDVMDLRIHA
                     FGLIELLTRIPMMVSAGIGGDLVRFALVSIVFFGVNYGLFRVLIVKFKLPTPGRMGNY
                     LDESSESMSEDEKMDIIIQNLGGRANIVEIDACMTRLRITVNDPQLVAEYALWKPTGA
                     LGAVIKEQGVQVIYGPGVDVIKSRLVEKFSAQPA"
     CDS             5513..6310
                     /codon_start=1
                     /product="Endonuclease/exonuclease/phosphatase family
                     protein"
                     /translation="MTLNTHSWQEEKQLEKLDVVAQAIIEQGCDVVALQEVNQHQNSS
                     AVDANILTNHTVLADNYGYLLQKKLMEYGYHYQLTWDFVHQSYDVYQEGLAFLTRLPI
                     VEHEVIDLSDNYDVNFWKHRRAVRIKVTSQRGDFNLYNCHCGWWSDSESSFEDQFNRI
                     KATLSTELSFLLGDFNNPSHIRNEGYDYVLQCGLIDCYEIAEIKDSGTTVIKNIDGWE
                     QNSQALRIDLVLSNQPMVVKQHQVIFNNDFYPVVSDHFGVLVEVDII"
     repeat_region   complement(6332..6354)
                     /gene="IRR_IS1F"
     CDS             complement(6347..6850)
                     /gene="insB"
                     /codon_start=1
                     /product="IS1F transposase InsB"
                     /translation="MSRQCTHYGRWPQHGFTSLKKLRPQSVTWRIQPGSDVIVCAEMD
                     EQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDG
                     WPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
                     LNIKHYQ"
     CDS             complement(6769..7044)
                     /gene="insA"
                     /codon_start=1
                     /product="IS1F transposase InsA"
                     /translation="-
                     ASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDM
                     AMNGVGCRASARIMGVGLNTVLRHLKNSGRSR"
     repeat_region   complement(7077..7099)
                     /gene="IRL_IS1F"
ORIGIN      
        1 ggtaatgact ccaacttact gatagtgttt tatgttcaga taatgcccga tgaccttgtc
       61 atgcagctcc accgattttg agaacgacag tgacttccgt ccaagccttg ccagatgttg
      121 tctcagattc aggttatgtc gctcaatgcg ctgagtgtaa cgcttgctga taacgtgcag
      181 ctttcccttc aggcgtgatt catacagcgg ccagccatcc gtcatccata ccacgacctc
      241 aaaggccgac agcaggctca gaagacgctc cagtgtggcc agagtgcgtt caccgaagac
      301 gtgcgccaca accgtcctcc gtatcctgtc atacgcgtaa aacagccagc gctgacgtga
      361 tttagcaccg acgtagcccc actgttcgtc catttcagcg cagacaatca catcactgcc
      421 cggttgtatg cgccaggtta ccgactgcgg cctgagtttt ttaagtgacg taaaaccgtg
      481 ttgaggccaa cgcccataat gcgtgcactg gcgcgacatc cgacgccatt catggccata
      541 tcaatgattt tctggtgcgt accgggctga gaggcggtgt aagtgaactg tagttgccat
      601 gttttacggc aatgagagca gagatagcgc tgatgtccgg cagtgctttt gccgttacgc
      661 accacgcctt cagtagcgga gcaggaagga catctgatgg aaatggaagc cacgcaagca
      721 ccttaaaatc accatcatac actaaatcag taagttggca gcattaccgc tcgctccttg
      781 gtcttgagtt caggtctaaa tcttgtgagt gaaaattaaa cactcatacg tccacccagt
      841 tttatttact gtgttgttag ccaatatatt ggatactatg cccaagtgac tcaactctga
      901 attctgtatc ttgaagtcac ttggagatat ggcaatagtg cattattgaa ggaaataggt
      961 aatgagtaaa aaaatgacca tggctgagat ttcaaggcag cttggaattt caacaatgac
     1021 tgtgtcgcgt tactttaatg gcggttatgt ctcggaagag aaccgcctta aaattgatgc
     1081 catcgtcaaa gagagtcatt acacgcctaa tatatttgct cgctccattc gtagccaatc
     1141 caatattatt ggtttcatcg cgcctcgtat tgagtcatat acgaccagtt tagtgattaa
     1201 aggggcgctg gctgctgcca atgaatcaca agtacgaatg ctgtttcatg ccacagggtt
     1261 taaccatgag tcagaatgcc aagctgtgag agagtttaac ggcttaaatg cattgggcac
     1321 gattattatt gcgtcaaagc acagtgtcga agagactttt tatcgaacct tagacaatgt
     1381 ggtatttatt ggcaaaaata ccccccacca ttgctgttta tattaccccg atcatgcagc
     1441 gattaaagcc ctcgtctccc acaccatcac tcagttaaaa cagcagcagg caccgctggc
     1501 tgccgtacat tatatttatg atgagcgtat gctttctagc cgcactaagt tgatgcaagc
     1561 cactattacc gacaccgtcc ctgagttgaa tttatcacta caagccctag ccaactcgga
     1621 ggacaaagct ggctatcagg ccgttcaatt acagcctaat catgtctact tttgtgccac
     1681 cgataatatt gccattcgtt tatatcgtcg cgccaaagaa cagcaactca agatcggtca
     1741 taacgtatgg attgtaggca tgggtgacta tgactacagc gatttgttag tcccgagcct
     1801 gacgactgtc gcttttaact actatcaagt gggctaccaa gctgtcaaaa aattgctcaa
     1861 gcgcgatttc agttctgttg aaggcacatt cgacataaag atgagagaaa gctcacaatt
     1921 cctctgaaat tgtgttgagt ccaaatcggt tctacttaac tatatatgtt aacgataaca
     1981 catatagtat aaccgatgac actcgctcct aatatgcact caaaaccact cgcgtggtgg
     2041 aagaaagcaa ccgcctacca aatttatccc cgtagttttt atgatacaaa ccaggacggt
     2101 attggtgaca tcaatggcat cattgctaag ctcgattatc ttgctgattt gggcattgat
     2161 ttaatatgga tttgcccatt ctatgcatca cctaatgatg ataacgggta cgatattagt
     2221 gactatcaag cgattcatcc tgactttgga acccttgagg atgtggatga actaatcagt
     2281 gctgctcata aacgtggcat tcgcattatt atggatttag tgatcaacca cactagcgac
     2341 gagcacccat ggttcattga atctcgagat aacaaaaata gccctaagcg cgattggtat
     2401 atttggcgtg atggttgtga gcctaccgct gaaaaaccga cttgcgaccc aaacaactgg
     2461 gaaagtattt ttcatggcag cgcatgggaa tatgataaaa aaactgcaca gtactacttg
     2521 cacctatttt caaaaaaaca acctgatctg aattgggaaa atcctgaagt taaacaagca
     2581 ctatttaaaa tgatttattg gtggttggag cgtggtattg atggcttccg tgtggatgca
     2641 attagccata ttcagaaaca accaggttta ccttctttac ccaatccaaa gggtgaaaag
     2701 tatgtctctt cgtttgatta tcatatgaat gtggcaggga ttgaaaaaca tctgcatgag
     2761 ttaaaagagc aggcgtttga tccctttaat atagtgacag ttggtgaggc taatggggtg
     2821 actgccgaca atgcattact ttgggttgcc gaagcgaccg agcacgatca aggcggtgtt
     2881 ttcaacatga tttttcagtt tgaacacatg aagctatggt cggaagaaca aagcaacgtg
     2941 cctttagatc tagtggagtt caaacgcatt ttatcgcgtt ggcaagatgc attagaaggt
     3001 aaaggctgga atgcgcttta tttagaaaac cacgatcaag ctcgtagcat tgatactttt
     3061 gccgacccta cctctaggta tgccagtgct accgcgttag cgagttgtta tttcttgatg
     3121 aaaggaactc cgtttattta tcagggccaa gagattggta tggtaaatca tcagtttact
     3181 tctcttgatg aatttaatga tgtgtctgct agaaatttaa ttcaaaagct atctcaacaa
     3241 ggtcaaagcg atgcagacat cttagccttg ttaaatcaag tatctcgcga ccacagtcgc
     3301 ttgccaatgc aatggaatgc gcaggatttt gccggttttt ctgaggtgca accttggttc
     3361 tccgtcaatc aacagtacgc agatatcaat gttgagcaac aacagaaaga tccaaattcg
     3421 attttgagtt tctataagca gctgattgct ctgcgtaagt caaatgtaag tttagtggtt
     3481 ggccgctatc aattgctgct acctaacgac cctaatatct atgcttatca acgtgttgca
     3541 caagatatga catggaccat tattaccaat ttaagtccac aagagagcat cgttgatatt
     3601 gaccgcatgc aattaggcga gttaatgctt gataatcaga atgtcaacaa tgaacacgtt
     3661 cgctcagatt ttatagcgac gcaaattgcc ccaccttatg ctgcgtatat atttgcaaga
     3721 aagcactaaa ttatatttat cgcagggcct tgataaggct accctgcatt taaaccaagg
     3781 aatcatttat gagtcagtta ctatcttttg atttttggca acgatttggg aaatccctta
     3841 tcgttgttat cgcggtgatg ccagccgctg gtatcatgat atcactcggt aaagttgtcg
     3901 caatgtatgc tggtggtatt ggtgccattg aaacactagg cgctattatg gaaaacatag
     3961 gctgggggat catagttaat cttcacctat tatttgctgt cgctattggt ggatcatggg
     4021 ctaaagaacg tgcaggagga gcatttgccg cgctgatcgc cttcatctta attaaccgta
     4081 tcactggggt tatattaggc gtggataacg ctatgctcag tgatcccgag gcggttgtga
     4141 tgagcctatt tggttctgag ctacctgtct ctcaattctt cactaatatt ttgggtgctc
     4201 ctgctctaaa tatgggtgtg tttatcggta ttatgtctgg ctatttaggt gccaacttat
     4261 ttaaccgtta tcatgacttc gctcgcttac ctcaggcatt agcatttttc aacggtaagc
     4321 gttttgtacc ttttgttgtt attttctatt cgatgattat tgcgtttgct ttatcaatcg
     4381 tatggccttt aattcaaggt gcgcttaatg attttggtca atggattgca acctctaaag
     4441 acactgcgcc gatcactgct cctttcttgt atggtacgtt agagcgctta ttactgccat
     4501 ttggtttaca ccacacttta actattccaa tgaattacac cgagttgggt ggtacttatg
     4561 agcttttaac aggggtgaat gcgggggcaa gtgtttatgg tcaagatcca ctatggcttg
     4621 cttgggttag cgatttaaat aatcttaaat taagcgatcc gacaacttac caacatctat
     4681 tagatacggt gcatccagca cgttttaaag cgggccaagt tattatcgca gcgtcttcat
     4741 tgattggtat tggacttgcg atgtatcact gtgttgatag tgataagcgc gcacaataca
     4801 agccaatgtt tttatctgcg tgtcttgctg tgttattaac cggtgtgaca gagcctattg
     4861 aattcatgtt tatgtttatt gctcctgttt tgtatgttgc gcatgcggta ttaacaggga
     4921 ttgcatttgc gttggtcgat gtgatggacc tacgtataca tgcctttggt ttgattgagt
     4981 tactcactcg tatacctatg atggtttcag cgggaatcgg tggtgatttg gttcgcttcg
     5041 cattggtttc tattgttttc tttggtgtta actatggatt attccgtgtg ctcattgtca
     5101 aatttaaatt gccaacaccg ggccgtatgg gtaactacct cgatgaaagt tcagaatcca
     5161 tgtcagaaga tgagaagatg gacattatta ttcagaattt aggtggccgt gccaatattg
     5221 ttgagattga tgcttgtatg actcgcttgc gcattacagt taatgacccg cagttggttg
     5281 ctgaatatgc attatggaag ccgacaggag cacttggtgc tgtaattaaa gaacagggag
     5341 tacaagttat ttatggtcca ggagtggatg tcattaaatc tagattagtt gagaagtttt
     5401 cagcacaacc tgcgtaattc attctctacc ctagataaaa taacctcgag aaacgcagcc
     5461 ttgagctgcg tttaactcgc tacaatgaat aagtgaataa taaatgaagt taatgacgct
     5521 aaatacccat agttggcaag aagagaaaca actagaaaag ttggatgtgg tagcgcaagc
     5581 cattatcgag caagggtgcg atgtcgttgc attacaggaa gtgaatcaac atcaaaatag
     5641 ctcggctgtt gatgccaata tattaactaa tcacaccgtg ctcgctgata actatggata
     5701 tttattacag aaaaaactca tggaatacgg ctatcactac caacttacat gggatttcgt
     5761 tcatcaaagt tatgacgtat atcaagaggg actggcattc ttgacgcgac tgccgattgt
     5821 cgagcatgag gtcattgacc taagtgataa ctatgatgtg aacttttgga aacataggcg
     5881 agcagtgcgt attaaggtca cttctcaacg aggtgatttc aatctttata attgtcattg
     5941 cggctggtgg agtgactcag agagctcatt tgaggaccag ttcaacagaa taaaggcaac
     6001 attatcaaca gagttgagct ttctattagg cgatttcaac aacccaagcc atatacgcaa
     6061 tgaaggttat gattatgtat tacagtgcgg tttgatagat tgttatgaaa tagcagaaat
     6121 aaaagactcc ggaactactg taattaaaaa tattgacggt tgggaacaaa atagccaagc
     6181 gctacgcatt gatttggttc ttagtaatca accgatggtg gtaaagcagc atcaggtgat
     6241 ttttaataac gatttttatc cggttgtatc ggatcacttt ggggttctgg tggaagtaga
     6301 cattatttaa tgtttattca tagactaaac cggtaatgac tccaacttac tgatagtgtt
     6361 ttatgttcag ataatgcccg atgaccttgt catgcagctc caccgatttt gagaacgaca
     6421 gtgacttccg tcccagcctt gccagatgtt gtctcagatt caggttatgt cgctcaatgc
     6481 gctgagtgta acgcttgctg ataacgtgca gctttccctt caggcgtgat tcatacagcg
     6541 gccagccatc cgtcatccat accacgacct caaaggccga cagcaggctc agaagacgct
     6601 ccagtgtggc cagagtgcgt tcaccgaaga cgtgcgccac aaccgtcctc cgtatcctgt
     6661 catacgcgta aaacagccag cgctgacgtg atttagcacc gacgtagccc cactgttcgt
     6721 ccatttcagc gcagacaatc acatcactgc ccggttgtat gcgccaggtt accgactgcg
     6781 gcctgagttt tttaagtgac gtaaaaccgt gttgaggcca acgcccataa tgcgtgcact
     6841 ggcgcgacat ccgacgccat tcatggccat atcaatgatt ttctggtgcg taccgggctg
     6901 agaggcggtg taagtgaact gtagttgcca tgttttacgg caatgagagc agagatagcg
     6961 ctgatgtccg gcagtgcttt tgccgttacg caccacgcct tcagtagcgg agcaggaagg
     7021 acatctgatg gaaatggaag ccacgcaagc accttaaaat caccatcata cactaaatca
     7081 gtaagttggc agcattacc
//
